Ascorbic acid, dehydroascorbic acid and glutathione in liver disease.

Efficient transport and accumulation of vitamin C in HL-60 cells depleted of glutathione.

Human myeloid leukemia cells (HL-60) transport only the oxidized form of vitamin C (dehydroascorbic acid) and accumulate the vitamin in the reduced form, ascorbic acid. We performed a detailed study of the role of glutathione in the intracellular trapping/accumulation of ascorbic acid in HL-60 cells. Uptake studies using HL-60 cells depleted of glutathione by treatment with L-buthionine-(S,R) s...

Reaction between dehydroascorbic acid and dialysed lens extract in vitro.

IT is generally accepted that the high concentration of ascorbic acid in the aqueous humour in some species is due to active transfer of this substance from the plasma into the eye by the ciliary epithelium. However, the possibility of ascorbic acid formation in the eye has not been disproved, and Barany and Langham (1955) stated that, in the rabbit, a concentration of 10-14 mg./ml. ascorbic ac...

Reaction between Dehydroascorbic Acid and Dialysed Lens Extract

IT is generally accepted that the high concentration of ascorbic acid in the aqueous humour in some species is due to active transfer of this substance from the plasma into the eye by the ciliary epithelium. However, the possibility of ascorbic acid formation in the eye has not been disproved, and Barany and Langham (1955) stated that, in the rabbit, a concentration of 10-14 mg./ml. ascorbic ac...

Consequences of the Combined α-tocopherol, Ascorbic Acid and α-lipoic Acid on the Glutathione, Cholesterol and Fatty Acid Composition in Muscle and Liver of Diabetic Rats

Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent.

Vitamin C plays an important role in neutralizing toxic free radicals formed during oxidative metabolism or UV exposure of human skin. This study was performed to investigate the mechanisms that regulate the homoeostasis of vitamin C in HaCaT cells by identifying the events involved in the transport and in the reduction of dehydroascorbic acid. Dehydroascorbic acid accumulated to a greater exte...